Culture Conditions
All cultures in the CPCC are grown in defined media (see Culture Media). Information on the appropriate medium and the complete history for each isolate is available from the Curator.
Cultures are grown in controlled environment chambers primarily at 20°C; a small number of strains are kept at 10°C. All are grown under low lighting using fluorescent Cool-White or Daylight lamps and a Day:Night cycle of 12:12. All cultures are currently maintained by serial transfer. Duplicate cultures are kept in a back-up environmental chamber for each of the two temperatures in use.
We are using cryopreservation in liquid nitrogen for some of the valuable isolates to further ensure their long-term maintenance and genetic stability.
More detailed culturing information will be available online in the future.